Review



human procollagen iα1 duoset elisa kit  (R&D Systems)


Bioz Verified Symbol R&D Systems is a verified supplier
Bioz Manufacturer Symbol R&D Systems manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 96

    Structured Review

    R&D Systems human procollagen iα1 duoset elisa kit
    Human Procollagen Iα1 Duoset Elisa Kit, supplied by R&D Systems, used in various techniques. Bioz Stars score: 96/100, based on 130 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+procollagen/Human+Pro-Collagen+I+alpha+1+DuoSet+ELISA/pm41751890-246-1-8
    Average 96 stars, based on 130 article reviews
    human procollagen iα1 duoset elisa kit - by Bioz Stars, 2026-09
    96/100 stars

    Images

    Related Articles

    Cell Culture:

    Article Title: Injectable mineralized Sr-hydroxyapatite nanoparticles-loaded ɛ-polylysine-hyaluronic acid composite hydrogels for bone regeneration.
    Article Snippet: For the antibacterial studies: Escherichia coli (E. coli, American Type Culture Collection (ATCC©) 25922TM) methicillin-sensitive Staphylococcus aureus (S. aureus, ATCC© 25923TM), extended-spectrum β-lactamase E. coli (ESBL E. coli, clinically isolated from patients at Riga East Clinical University Hospital, Latvia; ethical committee approval ID: 2- PĒK-4/462/2022), methicillin-resistant S. aureus (MRSA, clinically isolated from patients at Riga East Clinical University Hospital, Latvia; ethical committee approval ID: 2-PĒK-4/462/2022), tryptone soya broth (TSB, CM0129, Oxoid Limited, Hampshire, United Kingdom), tryptone soya agar (TSA, casein soya bean digest agar, Code: CM0131, Oxoid Limited, Hampshire, United Kingdom). .. For the cell culture studies: human osteoblast line MG-63 (Cat.No CRL-1427) and mouse preosteoblast cell line MC3T3-E1 subclone 4 (Cat. No CRL-2593) were purchased from American Type Culture Collection (ATCC, Manassas, Virginia, USA), Dulbecco’s modified Eagle’s medium (DMEM, Sigma, Irvine, UK), fetal bovine serum (FBS, Sigma, St. Louis, MO, USA), penicillin/streptomycin solution (Sigma, St. Louis, MO, USA), alpha-modified Eagle’s medium with nucleosides and without vitamin C (α-MEM, without L-ascorbic acid, Gibco, Grand Island, NY, USA), trypsin/EDTA (Sigma, St. Louis, MO, USA), phosphate-buffered saline (PBS, Sigma, Irvine, UK), 3-[4,5-dimethylthiazol-2-yl]-2,5 diphenyl tetrazolium bromide (MTT, Sigma, St. Louis, MO, USA), dimethyl sulfoxide (DMSO, Sigma, St. Louis, MO, USA), Alkaline Phosphatase Assay Kit (Sigma, St. Louis, MO, USA), Triton X-100 (Sigma, St. Louis, MO, USA), bovine serum albumin (BSA, Sigma, Irvine, UK), QuickStart Bradford 1× Dye Reagent (BioRad, Hercules, CA, USA), human procollagen I DuoSet ELISA kit (R&D Systems, Minneapolis, MN, USA), human matrix metalloproteinase 1 DuoSet ELISA kit (MMP-1, R&D Systems, Minneapolis, MN, USA). .. Cell viability dyes, SYTO 9 and propidium iodide were supplied by Life Technologies Corp. (Eugene, OR, USA).

    Modification:

    Article Title: Injectable mineralized Sr-hydroxyapatite nanoparticles-loaded ɛ-polylysine-hyaluronic acid composite hydrogels for bone regeneration.
    Article Snippet: For the antibacterial studies: Escherichia coli (E. coli, American Type Culture Collection (ATCC©) 25922TM) methicillin-sensitive Staphylococcus aureus (S. aureus, ATCC© 25923TM), extended-spectrum β-lactamase E. coli (ESBL E. coli, clinically isolated from patients at Riga East Clinical University Hospital, Latvia; ethical committee approval ID: 2- PĒK-4/462/2022), methicillin-resistant S. aureus (MRSA, clinically isolated from patients at Riga East Clinical University Hospital, Latvia; ethical committee approval ID: 2-PĒK-4/462/2022), tryptone soya broth (TSB, CM0129, Oxoid Limited, Hampshire, United Kingdom), tryptone soya agar (TSA, casein soya bean digest agar, Code: CM0131, Oxoid Limited, Hampshire, United Kingdom). .. For the cell culture studies: human osteoblast line MG-63 (Cat.No CRL-1427) and mouse preosteoblast cell line MC3T3-E1 subclone 4 (Cat. No CRL-2593) were purchased from American Type Culture Collection (ATCC, Manassas, Virginia, USA), Dulbecco’s modified Eagle’s medium (DMEM, Sigma, Irvine, UK), fetal bovine serum (FBS, Sigma, St. Louis, MO, USA), penicillin/streptomycin solution (Sigma, St. Louis, MO, USA), alpha-modified Eagle’s medium with nucleosides and without vitamin C (α-MEM, without L-ascorbic acid, Gibco, Grand Island, NY, USA), trypsin/EDTA (Sigma, St. Louis, MO, USA), phosphate-buffered saline (PBS, Sigma, Irvine, UK), 3-[4,5-dimethylthiazol-2-yl]-2,5 diphenyl tetrazolium bromide (MTT, Sigma, St. Louis, MO, USA), dimethyl sulfoxide (DMSO, Sigma, St. Louis, MO, USA), Alkaline Phosphatase Assay Kit (Sigma, St. Louis, MO, USA), Triton X-100 (Sigma, St. Louis, MO, USA), bovine serum albumin (BSA, Sigma, Irvine, UK), QuickStart Bradford 1× Dye Reagent (BioRad, Hercules, CA, USA), human procollagen I DuoSet ELISA kit (R&D Systems, Minneapolis, MN, USA), human matrix metalloproteinase 1 DuoSet ELISA kit (MMP-1, R&D Systems, Minneapolis, MN, USA). .. Cell viability dyes, SYTO 9 and propidium iodide were supplied by Life Technologies Corp. (Eugene, OR, USA).

    Saline:

    Article Title: Injectable mineralized Sr-hydroxyapatite nanoparticles-loaded ɛ-polylysine-hyaluronic acid composite hydrogels for bone regeneration.
    Article Snippet: For the antibacterial studies: Escherichia coli (E. coli, American Type Culture Collection (ATCC©) 25922TM) methicillin-sensitive Staphylococcus aureus (S. aureus, ATCC© 25923TM), extended-spectrum β-lactamase E. coli (ESBL E. coli, clinically isolated from patients at Riga East Clinical University Hospital, Latvia; ethical committee approval ID: 2- PĒK-4/462/2022), methicillin-resistant S. aureus (MRSA, clinically isolated from patients at Riga East Clinical University Hospital, Latvia; ethical committee approval ID: 2-PĒK-4/462/2022), tryptone soya broth (TSB, CM0129, Oxoid Limited, Hampshire, United Kingdom), tryptone soya agar (TSA, casein soya bean digest agar, Code: CM0131, Oxoid Limited, Hampshire, United Kingdom). .. For the cell culture studies: human osteoblast line MG-63 (Cat.No CRL-1427) and mouse preosteoblast cell line MC3T3-E1 subclone 4 (Cat. No CRL-2593) were purchased from American Type Culture Collection (ATCC, Manassas, Virginia, USA), Dulbecco’s modified Eagle’s medium (DMEM, Sigma, Irvine, UK), fetal bovine serum (FBS, Sigma, St. Louis, MO, USA), penicillin/streptomycin solution (Sigma, St. Louis, MO, USA), alpha-modified Eagle’s medium with nucleosides and without vitamin C (α-MEM, without L-ascorbic acid, Gibco, Grand Island, NY, USA), trypsin/EDTA (Sigma, St. Louis, MO, USA), phosphate-buffered saline (PBS, Sigma, Irvine, UK), 3-[4,5-dimethylthiazol-2-yl]-2,5 diphenyl tetrazolium bromide (MTT, Sigma, St. Louis, MO, USA), dimethyl sulfoxide (DMSO, Sigma, St. Louis, MO, USA), Alkaline Phosphatase Assay Kit (Sigma, St. Louis, MO, USA), Triton X-100 (Sigma, St. Louis, MO, USA), bovine serum albumin (BSA, Sigma, Irvine, UK), QuickStart Bradford 1× Dye Reagent (BioRad, Hercules, CA, USA), human procollagen I DuoSet ELISA kit (R&D Systems, Minneapolis, MN, USA), human matrix metalloproteinase 1 DuoSet ELISA kit (MMP-1, R&D Systems, Minneapolis, MN, USA). .. Cell viability dyes, SYTO 9 and propidium iodide were supplied by Life Technologies Corp. (Eugene, OR, USA).

    MTT Assay:

    Article Title: Injectable mineralized Sr-hydroxyapatite nanoparticles-loaded ɛ-polylysine-hyaluronic acid composite hydrogels for bone regeneration.
    Article Snippet: For the antibacterial studies: Escherichia coli (E. coli, American Type Culture Collection (ATCC©) 25922TM) methicillin-sensitive Staphylococcus aureus (S. aureus, ATCC© 25923TM), extended-spectrum β-lactamase E. coli (ESBL E. coli, clinically isolated from patients at Riga East Clinical University Hospital, Latvia; ethical committee approval ID: 2- PĒK-4/462/2022), methicillin-resistant S. aureus (MRSA, clinically isolated from patients at Riga East Clinical University Hospital, Latvia; ethical committee approval ID: 2-PĒK-4/462/2022), tryptone soya broth (TSB, CM0129, Oxoid Limited, Hampshire, United Kingdom), tryptone soya agar (TSA, casein soya bean digest agar, Code: CM0131, Oxoid Limited, Hampshire, United Kingdom). .. For the cell culture studies: human osteoblast line MG-63 (Cat.No CRL-1427) and mouse preosteoblast cell line MC3T3-E1 subclone 4 (Cat. No CRL-2593) were purchased from American Type Culture Collection (ATCC, Manassas, Virginia, USA), Dulbecco’s modified Eagle’s medium (DMEM, Sigma, Irvine, UK), fetal bovine serum (FBS, Sigma, St. Louis, MO, USA), penicillin/streptomycin solution (Sigma, St. Louis, MO, USA), alpha-modified Eagle’s medium with nucleosides and without vitamin C (α-MEM, without L-ascorbic acid, Gibco, Grand Island, NY, USA), trypsin/EDTA (Sigma, St. Louis, MO, USA), phosphate-buffered saline (PBS, Sigma, Irvine, UK), 3-[4,5-dimethylthiazol-2-yl]-2,5 diphenyl tetrazolium bromide (MTT, Sigma, St. Louis, MO, USA), dimethyl sulfoxide (DMSO, Sigma, St. Louis, MO, USA), Alkaline Phosphatase Assay Kit (Sigma, St. Louis, MO, USA), Triton X-100 (Sigma, St. Louis, MO, USA), bovine serum albumin (BSA, Sigma, Irvine, UK), QuickStart Bradford 1× Dye Reagent (BioRad, Hercules, CA, USA), human procollagen I DuoSet ELISA kit (R&D Systems, Minneapolis, MN, USA), human matrix metalloproteinase 1 DuoSet ELISA kit (MMP-1, R&D Systems, Minneapolis, MN, USA). .. Cell viability dyes, SYTO 9 and propidium iodide were supplied by Life Technologies Corp. (Eugene, OR, USA).

    ALP Assay:

    Article Title: Injectable mineralized Sr-hydroxyapatite nanoparticles-loaded ɛ-polylysine-hyaluronic acid composite hydrogels for bone regeneration.
    Article Snippet: For the antibacterial studies: Escherichia coli (E. coli, American Type Culture Collection (ATCC©) 25922TM) methicillin-sensitive Staphylococcus aureus (S. aureus, ATCC© 25923TM), extended-spectrum β-lactamase E. coli (ESBL E. coli, clinically isolated from patients at Riga East Clinical University Hospital, Latvia; ethical committee approval ID: 2- PĒK-4/462/2022), methicillin-resistant S. aureus (MRSA, clinically isolated from patients at Riga East Clinical University Hospital, Latvia; ethical committee approval ID: 2-PĒK-4/462/2022), tryptone soya broth (TSB, CM0129, Oxoid Limited, Hampshire, United Kingdom), tryptone soya agar (TSA, casein soya bean digest agar, Code: CM0131, Oxoid Limited, Hampshire, United Kingdom). .. For the cell culture studies: human osteoblast line MG-63 (Cat.No CRL-1427) and mouse preosteoblast cell line MC3T3-E1 subclone 4 (Cat. No CRL-2593) were purchased from American Type Culture Collection (ATCC, Manassas, Virginia, USA), Dulbecco’s modified Eagle’s medium (DMEM, Sigma, Irvine, UK), fetal bovine serum (FBS, Sigma, St. Louis, MO, USA), penicillin/streptomycin solution (Sigma, St. Louis, MO, USA), alpha-modified Eagle’s medium with nucleosides and without vitamin C (α-MEM, without L-ascorbic acid, Gibco, Grand Island, NY, USA), trypsin/EDTA (Sigma, St. Louis, MO, USA), phosphate-buffered saline (PBS, Sigma, Irvine, UK), 3-[4,5-dimethylthiazol-2-yl]-2,5 diphenyl tetrazolium bromide (MTT, Sigma, St. Louis, MO, USA), dimethyl sulfoxide (DMSO, Sigma, St. Louis, MO, USA), Alkaline Phosphatase Assay Kit (Sigma, St. Louis, MO, USA), Triton X-100 (Sigma, St. Louis, MO, USA), bovine serum albumin (BSA, Sigma, Irvine, UK), QuickStart Bradford 1× Dye Reagent (BioRad, Hercules, CA, USA), human procollagen I DuoSet ELISA kit (R&D Systems, Minneapolis, MN, USA), human matrix metalloproteinase 1 DuoSet ELISA kit (MMP-1, R&D Systems, Minneapolis, MN, USA). .. Cell viability dyes, SYTO 9 and propidium iodide were supplied by Life Technologies Corp. (Eugene, OR, USA).

    Enzyme-linked Immunosorbent Assay:

    Article Title: Injectable mineralized Sr-hydroxyapatite nanoparticles-loaded ɛ-polylysine-hyaluronic acid composite hydrogels for bone regeneration.
    Article Snippet: For the antibacterial studies: Escherichia coli (E. coli, American Type Culture Collection (ATCC©) 25922TM) methicillin-sensitive Staphylococcus aureus (S. aureus, ATCC© 25923TM), extended-spectrum β-lactamase E. coli (ESBL E. coli, clinically isolated from patients at Riga East Clinical University Hospital, Latvia; ethical committee approval ID: 2- PĒK-4/462/2022), methicillin-resistant S. aureus (MRSA, clinically isolated from patients at Riga East Clinical University Hospital, Latvia; ethical committee approval ID: 2-PĒK-4/462/2022), tryptone soya broth (TSB, CM0129, Oxoid Limited, Hampshire, United Kingdom), tryptone soya agar (TSA, casein soya bean digest agar, Code: CM0131, Oxoid Limited, Hampshire, United Kingdom). .. For the cell culture studies: human osteoblast line MG-63 (Cat.No CRL-1427) and mouse preosteoblast cell line MC3T3-E1 subclone 4 (Cat. No CRL-2593) were purchased from American Type Culture Collection (ATCC, Manassas, Virginia, USA), Dulbecco’s modified Eagle’s medium (DMEM, Sigma, Irvine, UK), fetal bovine serum (FBS, Sigma, St. Louis, MO, USA), penicillin/streptomycin solution (Sigma, St. Louis, MO, USA), alpha-modified Eagle’s medium with nucleosides and without vitamin C (α-MEM, without L-ascorbic acid, Gibco, Grand Island, NY, USA), trypsin/EDTA (Sigma, St. Louis, MO, USA), phosphate-buffered saline (PBS, Sigma, Irvine, UK), 3-[4,5-dimethylthiazol-2-yl]-2,5 diphenyl tetrazolium bromide (MTT, Sigma, St. Louis, MO, USA), dimethyl sulfoxide (DMSO, Sigma, St. Louis, MO, USA), Alkaline Phosphatase Assay Kit (Sigma, St. Louis, MO, USA), Triton X-100 (Sigma, St. Louis, MO, USA), bovine serum albumin (BSA, Sigma, Irvine, UK), QuickStart Bradford 1× Dye Reagent (BioRad, Hercules, CA, USA), human procollagen I DuoSet ELISA kit (R&D Systems, Minneapolis, MN, USA), human matrix metalloproteinase 1 DuoSet ELISA kit (MMP-1, R&D Systems, Minneapolis, MN, USA). .. Cell viability dyes, SYTO 9 and propidium iodide were supplied by Life Technologies Corp. (Eugene, OR, USA).



    Similar Products

    94
    Elabscience Biotechnology human type i procollagen elisa kit
    Scheme of the fabrication of the microcarriers and delivery of engineered nanovesicles for photoaging therapy. Abbreviations: HDF, human dermal fibroblast; Exos, exosomes; Emvs, engineered nanovesicles; HDF COL1A1 Emvs, Emvs derived from HDFs transfected with COL1A1 plasmid (COL1A1 mRNA–encapsulating nanovesicles); ROS, reactive oxygen species; COL1A1, collagen <t>type</t> <t>I</t> alpha 1 chain.
    Human Type I Procollagen Elisa Kit, supplied by Elabscience Biotechnology, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+procollagen/Human+PC%E2%85%A0+(Procollagen+%E2%85%A0)+ELISA+Kit/pmc13101773-154-11-17
    Average 94 stars, based on 1 article reviews
    human type i procollagen elisa kit - by Bioz Stars, 2026-09
    94/100 stars
      Buy from Supplier

    94
    Novus Biologicals piiinp
    LMS percentiles for <t>urinary</t> <t>MCP-1</t> (boys, a; girls, b) and (c) <t>PIIINP</t> to creatinine ratios in the HARP cohort. The 5th, 10th, 25th (dashed line), 50th (bold line), 75th (dashed line), 90th, and 95th percentiles are given. Data on boys and girls is given as blue triangles and orange dots, respectively. crea, creatinine; HARP, Hannover reference values for pediatrics; LMS, lambda-mu-sigma; MCP-1, monocyte chemoattractant protein-1; PIIINP, procollagen type III amino-terminal propeptide.
    Piiinp, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+procollagen/Human+Procollagen+type+III+N-terminal+Propeptide+ELISA+Kit+(Colorimetric)/pmc12968418-47-63-64
    Average 94 stars, based on 1 article reviews
    piiinp - by Bioz Stars, 2026-09
    94/100 stars
      Buy from Supplier

    96
    R&D Systems human procollagen iα1 duoset elisa kit
    LMS percentiles for <t>urinary</t> <t>MCP-1</t> (boys, a; girls, b) and (c) <t>PIIINP</t> to creatinine ratios in the HARP cohort. The 5th, 10th, 25th (dashed line), 50th (bold line), 75th (dashed line), 90th, and 95th percentiles are given. Data on boys and girls is given as blue triangles and orange dots, respectively. crea, creatinine; HARP, Hannover reference values for pediatrics; LMS, lambda-mu-sigma; MCP-1, monocyte chemoattractant protein-1; PIIINP, procollagen type III amino-terminal propeptide.
    Human Procollagen Iα1 Duoset Elisa Kit, supplied by R&D Systems, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+procollagen/Human+Pro-Collagen+I+alpha+1+DuoSet+ELISA/pm41751890-246-1-8
    Average 96 stars, based on 1 article reviews
    human procollagen iα1 duoset elisa kit - by Bioz Stars, 2026-09
    96/100 stars
      Buy from Supplier

    94
    Novus Biologicals serum procollagen type i n terminal propeptide
    Biomarkers. Fig. 1 depicts all assessed biomarker concentrations. The total hip, femoral neck, or lumbar spine (Lower) group is depicted in black filled circles, while the control (Con) group is in clear squares. A, P1NP (pg/mL); B, alkaline phosphatase (ALP) (pg/mL); C, tartrate-resistant acid phosphatase 5b (TRAP5b) (U/L); D, sclerostin (pmol/L); E, β C-terminal cross-linked telopeptide of <t>type</t> <t>I</t> collagen (βCTX) (pmol/L); F, testosterone (ng/mL); G, estradiol (pg/mL); H, parathyroid hormone (PTH) (pg/mL); I, insulin-like growth factor-1 (IGF-1) (ng/mL); J, insulin-like growth factor-1 binding protein 5 (IGFBP-5) (ng/mL).
    Serum Procollagen Type I N Terminal Propeptide, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+procollagen/Human+Procollagen+Type+1+N-Terminal+Propeptide+ELISA+Kit+(Colorimetric)/pmc12910377-91-12-19
    Average 94 stars, based on 1 article reviews
    serum procollagen type i n terminal propeptide - by Bioz Stars, 2026-09
    94/100 stars
      Buy from Supplier

    94
    Novus Biologicals propeptide 23
    Biomarkers. Fig. 1 depicts all assessed biomarker concentrations. The total hip, femoral neck, or lumbar spine (Lower) group is depicted in black filled circles, while the control (Con) group is in clear squares. A, P1NP (pg/mL); B, alkaline phosphatase (ALP) (pg/mL); C, tartrate-resistant acid phosphatase 5b (TRAP5b) (U/L); D, sclerostin (pmol/L); E, β C-terminal cross-linked telopeptide of <t>type</t> <t>I</t> collagen (βCTX) (pmol/L); F, testosterone (ng/mL); G, estradiol (pg/mL); H, parathyroid hormone (PTH) (pg/mL); I, insulin-like growth factor-1 (IGF-1) (ng/mL); J, insulin-like growth factor-1 binding protein 5 (IGFBP-5) (ng/mL).
    Propeptide 23, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+procollagen/Human+Procollagen+Type+1+N-Terminal+Propeptide+ELISA+Kit+(Colorimetric)/10__1210_slash_jendso_slash_bvag003-50-19-22
    Average 94 stars, based on 1 article reviews
    propeptide 23 - by Bioz Stars, 2026-09
    94/100 stars
      Buy from Supplier

    94
    Elabscience Biotechnology elisa kit
    Biomarkers. Fig. 1 depicts all assessed biomarker concentrations. The total hip, femoral neck, or lumbar spine (Lower) group is depicted in black filled circles, while the control (Con) group is in clear squares. A, P1NP (pg/mL); B, alkaline phosphatase (ALP) (pg/mL); C, tartrate-resistant acid phosphatase 5b (TRAP5b) (U/L); D, sclerostin (pmol/L); E, β C-terminal cross-linked telopeptide of <t>type</t> <t>I</t> collagen (βCTX) (pmol/L); F, testosterone (ng/mL); G, estradiol (pg/mL); H, parathyroid hormone (PTH) (pg/mL); I, insulin-like growth factor-1 (IGF-1) (ng/mL); J, insulin-like growth factor-1 binding protein 5 (IGFBP-5) (ng/mL).
    Elisa Kit, supplied by Elabscience Biotechnology, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+procollagen/Human+P%E2%85%A0NP+(Procollagen+%E2%85%A0+N-Terminal+Propeptide)+ELISA+Kit/pmc12842479-96-8-14
    Average 94 stars, based on 1 article reviews
    elisa kit - by Bioz Stars, 2026-09
    94/100 stars
      Buy from Supplier

    Image Search Results


    Scheme of the fabrication of the microcarriers and delivery of engineered nanovesicles for photoaging therapy. Abbreviations: HDF, human dermal fibroblast; Exos, exosomes; Emvs, engineered nanovesicles; HDF COL1A1 Emvs, Emvs derived from HDFs transfected with COL1A1 plasmid (COL1A1 mRNA–encapsulating nanovesicles); ROS, reactive oxygen species; COL1A1, collagen type I alpha 1 chain.

    Journal: Materials Today Bio

    Article Title: Microcarriers encapsulating COL1A1 mRNA-loaded nanovesicles for skin photoaging treatment

    doi: 10.1016/j.mtbio.2026.103126

    Figure Lengend Snippet: Scheme of the fabrication of the microcarriers and delivery of engineered nanovesicles for photoaging therapy. Abbreviations: HDF, human dermal fibroblast; Exos, exosomes; Emvs, engineered nanovesicles; HDF COL1A1 Emvs, Emvs derived from HDFs transfected with COL1A1 plasmid (COL1A1 mRNA–encapsulating nanovesicles); ROS, reactive oxygen species; COL1A1, collagen type I alpha 1 chain.

    Article Snippet: The concentration of human type I procollagen was measured using a human type I procollagen ELISA kit (Elabscience Biotechnology, China) according to the manufacturer's instructions.

    Techniques: Derivative Assay, Transfection, Plasmid Preparation

    In vivo biosafety evaluation. a) In vivo imaging of subcutaneous injection sites across different groups. b) Quantification of relative fluorescence intensity (n = 5). c) Histological analysis via H&E staining of the heart, liver, spleen, lung, and kidney tissues. d) ELISA quantification of IL-6 levels and TNF-α levels (n = 5). e) Quantification of ALT, AST, CREA, and UA levels (n = 3). The scale bar is 300 μm in c).

    Journal: Materials Today Bio

    Article Title: Microcarriers encapsulating COL1A1 mRNA-loaded nanovesicles for skin photoaging treatment

    doi: 10.1016/j.mtbio.2026.103126

    Figure Lengend Snippet: In vivo biosafety evaluation. a) In vivo imaging of subcutaneous injection sites across different groups. b) Quantification of relative fluorescence intensity (n = 5). c) Histological analysis via H&E staining of the heart, liver, spleen, lung, and kidney tissues. d) ELISA quantification of IL-6 levels and TNF-α levels (n = 5). e) Quantification of ALT, AST, CREA, and UA levels (n = 3). The scale bar is 300 μm in c).

    Article Snippet: The concentration of human type I procollagen was measured using a human type I procollagen ELISA kit (Elabscience Biotechnology, China) according to the manufacturer's instructions.

    Techniques: In Vivo, In Vivo Imaging, Injection, Fluorescence, Staining, Enzyme-linked Immunosorbent Assay

    LMS percentiles for urinary MCP-1 (boys, a; girls, b) and (c) PIIINP to creatinine ratios in the HARP cohort. The 5th, 10th, 25th (dashed line), 50th (bold line), 75th (dashed line), 90th, and 95th percentiles are given. Data on boys and girls is given as blue triangles and orange dots, respectively. crea, creatinine; HARP, Hannover reference values for pediatrics; LMS, lambda-mu-sigma; MCP-1, monocyte chemoattractant protein-1; PIIINP, procollagen type III amino-terminal propeptide.

    Journal: Kidney International Reports

    Article Title: Kidney Injury Urine Biomarker Normal Ranges in Children

    doi: 10.1016/j.ekir.2026.106348

    Figure Lengend Snippet: LMS percentiles for urinary MCP-1 (boys, a; girls, b) and (c) PIIINP to creatinine ratios in the HARP cohort. The 5th, 10th, 25th (dashed line), 50th (bold line), 75th (dashed line), 90th, and 95th percentiles are given. Data on boys and girls is given as blue triangles and orange dots, respectively. crea, creatinine; HARP, Hannover reference values for pediatrics; LMS, lambda-mu-sigma; MCP-1, monocyte chemoattractant protein-1; PIIINP, procollagen type III amino-terminal propeptide.

    Article Snippet: Urinary concentrations of KIM-1 (Quantikine ELISA, R&D system; MN Catalog No. DKM100; sensitivity 0.046 ng/ml), NGAL (Quantikine ELISA, R&D system; MN, Catalog No. DLCN20; sensitivity 0.04 ng/ml), DKK3 (Duo Set ELISA, R&D system; MN, Catalog No. DY1118, sensitivity 31.2 pg/ml), CHI3L1 (Quantikine ELISA, R&D system; MN, Catalog No. DC3L10; sensitivity 8.15 pg/ml), MCP-1 (Quantikine ELISA, R&D system;MN, Catalog No. DCP00; sensitivity 10 pg/ml), PIIINP (Novus Biologicals, Wiesbaden Nordenstadt, Germany, Catalog No. NBP2-76434; sensitivity 14.06 pg/ml), and EGF (Quantikine ELISA, R&D system; MN, Catalog No. DEG00; sensitivity 0.7 pg/ml) were measured using ELISA, according to the manufacturer’s protocols.

    Techniques:

    Biomarkers. Fig. 1 depicts all assessed biomarker concentrations. The total hip, femoral neck, or lumbar spine (Lower) group is depicted in black filled circles, while the control (Con) group is in clear squares. A, P1NP (pg/mL); B, alkaline phosphatase (ALP) (pg/mL); C, tartrate-resistant acid phosphatase 5b (TRAP5b) (U/L); D, sclerostin (pmol/L); E, β C-terminal cross-linked telopeptide of type I collagen (βCTX) (pmol/L); F, testosterone (ng/mL); G, estradiol (pg/mL); H, parathyroid hormone (PTH) (pg/mL); I, insulin-like growth factor-1 (IGF-1) (ng/mL); J, insulin-like growth factor-1 binding protein 5 (IGFBP-5) (ng/mL).

    Journal: Journal of the Endocrine Society

    Article Title: Subclinically low BMD in young men is associated with compromised bone microarchitecture and lower lean mass

    doi: 10.1210/jendso/bvag003

    Figure Lengend Snippet: Biomarkers. Fig. 1 depicts all assessed biomarker concentrations. The total hip, femoral neck, or lumbar spine (Lower) group is depicted in black filled circles, while the control (Con) group is in clear squares. A, P1NP (pg/mL); B, alkaline phosphatase (ALP) (pg/mL); C, tartrate-resistant acid phosphatase 5b (TRAP5b) (U/L); D, sclerostin (pmol/L); E, β C-terminal cross-linked telopeptide of type I collagen (βCTX) (pmol/L); F, testosterone (ng/mL); G, estradiol (pg/mL); H, parathyroid hormone (PTH) (pg/mL); I, insulin-like growth factor-1 (IGF-1) (ng/mL); J, insulin-like growth factor-1 binding protein 5 (IGFBP-5) (ng/mL).

    Article Snippet: Commercially available enzyme-linked immunosorbent assays were used to determine circulating concentrations of serum procollagen type I N-terminal propeptide (PINP; Novus catalog No. NBP2-76465, RRID:AB_3720306, 1:200 dilution, CV 5.3%, sensitivity 9.4 pg/mL), alkaline phosphatase (ALP; Novus catalog No. NBP2-68197, RRID:AB_3720307, CV 6.0%, sensitivity 46.9 pg/mL), tartrate-resistant acid phosphatase 5b (TRAP5b; Immunodiagnostic Systems catalog No. SB-TR201A, RRID:AB_3095995, CV 4.7%, sensitivity 0.4 U/L), sclerostin (Biomedica catalog No. BI-20492, RRID:AB_2894889, CV 6.4%, sensitivity 3.2 pmol/L), testosterone (Alpco Diagnostics catalog No. 11-TESHU-E01, RRID:AB_3720308, CV 4.2%, sensitivity 0.02 ng/mL), estradiol (Alpco Diagnostics catalog No. 11-ESTHU-E01, RRID:AB_2756385, CV 7.6%, sensitivity 10 pg/mL), insulin like growth factor-1 (IGF-1; Alpco Diagnostics catalog No. 22-IGFHU-E01, RRID:AB_2923276, 1:20 dilution, average CV 3.3%, assay sensitivity 9.4 pg/mL), IGF binding protein-5 (IGFBP-5; R and D Systems catalog No. DY875, RRID:AB_3720311, 3× dilution, CV 2.7%), parathyroid hormone (PTH; (Alpco Diagnostics catalog No. 21-IPTHU-E01, RRID:AB_2943035), CV 7.3%, sensitivity 1.6 pg/mL), and plasma β C-terminal cross-linked telopeptide of type I collagen (βCTX; Immunodiagnostic Systems catalog No. IS-3000N, RRID:AB_3720313, CV 6.2%, sensitivity 3.2 pmol/L).

    Techniques: Biomarker Discovery, Control, Binding Assay